TABLE 4.
Kinetic rates for FACS activity from P. putida CA-3 for a range of phenylalkanoic and alkanoic acidsa
| Acid substrate | Value for FadD from P. putida CA-3 |
||
|---|---|---|---|
| kcat (min−1) (mean ± SD) | Km (mM) (mean ± SD) | kcat/Km (min−1/mM−1) | |
| 5-Ph valeric | 24 ± 1 | 84 ± 2 | 286 |
| 8-Ph octanoic | 42 ± 1 | 79 ± 1 | 532 |
| 12-Ph dodecanoic | 112 ± 3 | 55 ± 1 | 2,036 |
| 15-Ph pentadecanoic | 108 ± 2 | 66 ± 2 | 1,636 |
| Valeric | 3 ± 1 | 32 ± 1 | 94 |
| Octanoic | 25 ± 1 | 26 ± 1 | 962 |
| Nonanoic | 15 ± 1 | 35 ± 2 | 429 |
| Myristic | 52 ± 2 | 46 ± 1 | 1,130 |
| Pentadecanoic | 61 ± 1 | 32 ± 1 | 1,906 |
| Palmitic | 124 ± 3 | 62 ± 2 | 2000 |
a
Ph, phenyl. All data are the averages of at least three determinations. All data have been rounded to the nearest whole number.