FIG. 1.

Schematic diagram of the oligonucleotide-gold nanoparticle aggregation assay and detection strategy. (A) Aggregation of the nanoparticle probes in the presence of HSP70 target DNA or RNA to form oligonucleotide-gold nanoparticle networks and the green to red color shift associated with this transition; (B) the sequence of the oligonucleotide-gold nanoparticle probes designed for detection of the HSP70 targets from C. parvum; (C) schematic diagram of the total internal reflection imaging setup used for detection of assay results.