TABLE 1.

Nucleic acid sequences of the probes used to functionalize gold nanoparticles for the aggregation assay and primers used to obtain target DNA and RNA

Probe or primer	Sequencec
Cryptosporidium probe A	5′-thiol-A15-PEG-ATCCTGTACGGCAGAGGATTG-3′a
Cryptosporidium probe B	5′-thiol-A15-PEG-AGCAACATCCAATAATAAGAG-3′
Cryptosporidium 42-ntb DNA	5′-CAATCCTCTGCCGTACAGGATCTCTTATTATTGGATGTTGCT-3′
Cryptosporidium 42-nt RNA	5′-CAAUCCUCUGCCGUACAGGAUCUCUUAUUAUUGGAUGUUGCU-3′
Cryptosporidium primers (DNA target)	5′-TCCTCTGCCGTACAGGATCTCTTA-3′ (1217 forward)
	5′-TGCTGCTCTTACCAGTACTCTTATCA-3′ (1537 reverse)
Entamoeba primers (control target)	5′-GAGAGCAGCAAAGAACCAAATTGC (175 forward)
	5′-TCTGCAGCAATCTTATCAGCA-3′ (1709 reverse)

^aPEG, polyethylene glycol.

^bnt, nucleotide.

^cThe numbers in parentheses correspond to the nucleotide number in the full sequence (HSP-0) a which the primer is located.