TABLE 1.
DNA extraction method characteristics
| Method (company) | Kit (company) | Protocol | Maximum no. of specimens/run | Elution buffer used |
|---|---|---|---|---|
| MagNA Pure Compact (Roche) | Nucleic acid isolation kit I (Roche) | Total nucleic acid plasma, 100-400 μla | 8 | NGc |
| BioRobot EZ1 (Qiagen) | EZ1 DNA tissue kit (Qiagen) | EZ1 DNA tissuea | 6 | Tris-EDTA |
| NucliSens easyMAG (bioMérieux) | NucliSens magnetic kit (bioMérieux) | Boom nucleic acid extractionb | 24 | 3 mM borate, pH 8.5 |
| Manual extraction | QIAamp DNA minikit (Qiagen) | Biological fluid | Sterile distilled water | |
| High Pure PCR template prepn kit (Roche) | Sterile distilled water |
a
A pretreatment using proteinase K is not included and must be done before starting extraction.
b
The lysis step is included in the protocol.
c
NG, not given.