TABLE 2.
Number of planned DNA extractions, Toxoplasma-specific PCR, inhibitor detections, and human beta-globin PCRs, according to the parasite concentration for each extraction method
| AF concn (T/ml) | No. of extractions done per method | No. of Toxoplasma PCRs |
No. of inhibitor detections |
No. of beta-globin quantifications |
||||
|---|---|---|---|---|---|---|---|---|
| Per extraction and per assay | Per assay | Per total no. of PCRs | Per extraction PCR | Per total no. of PCRs | Per extraction | Per total no. of PCRs | ||
| 0 | 6 | 1 | 6 | 12 | 1 | 6 | 1 | 6 |
| 1 | 6 | 8 | 48 | 96 | 1 | 6 | 1 | 6 |
| 2.5 | 3 | 8 | 24 | 48 | 1 | 3 | 1 | 3 |
| 5 | 3 | 6 | 18 | 36 | 1 | 3 | 1 | 3 |
| 25 | 2 | 4 | 8 | 16 | 1 | 2 | 1 | 2 |
| Total | 20 | 104 | 208 | 20 | 20 | |||