TABLE 2.
Comparison of the specificity of H3N8 and H7N7 subtype-specific rRT-PCR assays
| EIV subtype | EIV prototype strain and recent isolate | Virus titer (EID50/ml) | Detection of indicated subtype by rRT-PCRa |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| H3N8 |
H7N7 |
|||||||||
| EqFlu NP | EqFlu M | EqFlu HA3 | EqFlu HA3-Mia | EqFlu NP-Pra | EqFlu M-Pra | EqFlu HA7-Pra | EqFlu HA7 | |||
| H3N8 | A/equine/Miami/63 | 4.65 × 105 | + | + | − | + | − | − | − | − |
| A/equine/Alaska/91b | 2.14 × 109 | + | + | + | − | − | − | − | − | |
| A/equine/Kentucky/81b | 3.16 × 108 | + | + | + | − | − | − | − | − | |
| A/equine/Kentucky/02b | 2.15 × 107 | + | + | + | − | − | − | − | − | |
| A/equine/Ohio/03b | 3.16 × 107 | + | + | + | − | − | − | − | − | |
| A/equine/Newmarket/2/93c | 1 × 108 | + | + | + | − | − | − | − | − | |
| A/equine/Aboyne/05c | 6.81 × 103 | + | + | + | − | − | − | − | − | |
| A/equine/Richmond/07c | 4.65 × 105 | + | + | + | − | − | − | − | − | |
| H7N7 | A/equine/Prague/56 | 4.65 × 105 | − | − | − | − | + | + | + | + |
| A/equine/New York/73 | 4.7 × 107 | + | − | − | − | − | + | + | + | |
a
+, Nucleic acid was detectable by the rRT-PCR; −, nucleic acid was undetectable by the rRT-PCR.
b
American lineage.
c
Eurasian lineage.