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. 2009 Oct 21;47(12):3920–3926. doi: 10.1128/JCM.01288-09

TABLE 1.

Summary of the automated and manual DNA extraction methods used in this study

Extraction method Vendor Catalog no. Principle
MagNA Pure Compact Roche 03731146001 Lysis buffer and proteinase K treatments, followed by binding of nucleic acid to magnetic glass particles, magnetic separation of nucleic acid, and elution at high temperatures
MagNA Pure LC Roche 12236931001 Same as for the MagNA Pure Compact instrument
IT 1-2-3 DNA sample purification kit Idaho Technology 3800 Lysis buffer and bead beating, followed by binding of DNA to filter membrane, and contaminants pass through spin column
MasterPure complete DNA and RNA purification kit Epicentre MC85200 Lysis buffer and proteinase K treatments, followed by RNase A treatment, protein precipitation, and DNA precipitation
QIAamp DNA blood mini kit Qiagen 51106 Proteinase K treatment, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column
UltraClean microbial DNA isolation kit MoBio 12224-250 Lysis buffer and bead beating, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column