TABLE 4.
Real-time PCR limit of detection by the use of DNA extracted from spiked swabs
| Extraction methoda | LOD using DNA from indicated swabb |
||
|---|---|---|---|
| Polyester | Polyurethane foam | Rayon | |
| MagNA Pure Compact | 104 (50) | 105 (500) | 104 (50) |
| IT 1-2-3 | 105 (500) | 105 (500) | 106 (5,000) |
| MasterPure | 104 (50) | 104 (50) | 105 (500) |
| QIAamp | 105 (500) | 106 (5,000) | 105 (500) |
| UltraClean | 105 (500) | 105 (500) | 104 (50) |
a
Extraction methods were performed on samples recovered from triplicate swabs spiked with 10-fold dilutions of B. melitensis at a starting concentration of 107 CFU/ml.
b
The limit of detection (LOD) was determined to be the lowest concentration at which three out of three replicates produced a positive result (CFU/ml [CFU/reaction]).