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. 2009 Oct 21;47(12):3945–3951. doi: 10.1128/JCM.01601-09

FIG. 3.

FIG. 3.

(A) Results of PCR amplification of a 166-bp fragment with T. cruzi nuclear repetitive region-specific primers cruzi1 and cruzi2 extracted from DNA. Lane 1, patient sample 1; lane 2, patient sample 2; lane 3, patient sample 123; lane 4, patient sample 220; lane 5, patient sample 72; lane 6, positive control T. cruzi I strain Dm11; lane 7, positive control T. cruzi II strain VS; lane 8, molecular weight marker. (B) Results of PCR amplification of a 330-bp fragment with T. cruzi minicircle-specific primers 121 and 122 extracted from DNA. Lane 1, molecular weight marker; lane 2, patient sample 1; lane 3, patient sample 2; lane 4, patient sample 123; lane 5, positive control T. cruzi II strain VS; lane 6, positive control T. cruzi I strain Dm11; lane 7, negative control 1; lane 8, negative control 2. (C) Results of PCR amplification of a 110-bp fragment of the human β-globin region as an internal control. Lane 1, patient sample 1; lane 2, patient sample 2; lane 3, patient sample 123; lane 4, patient sample 200; lane 5, patient sample 127; lane 6, patient sample 122; lane 7, patient sample 256; lane 8, molecular weight marker.