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. 2009 Oct 21;47(12):4021–4028. doi: 10.1128/JCM.00504-09

TABLE 1.

Oligonucleotide primers used for sequencing of the H. pylori cagA gene

Primera Sequence Corresponding DNA sequenceb
cagA L2 (+) 5′-AAGGAGAAACAATGACTAACGAAACTATTG-3′ −11-19c
cagA L2 (−) 5′-TCCTTTAAGATTTTTGGAAACCACCTTTTG-3′ +4-3536
cagA B1 (−)* 5′-CTGCAAAAGATTGTTTGGCAGA-3′ 520-499
cagA M2 (+)* 5′-ATACAAGGCTTACCGCCTG-3′ 754-772
cagA S2* 5′-GGCAATGGTGGTCCTGGAGCTAGGC-3′ 976-1000
cagA M1 (−)* 5′-GTAGCCACATTGTCGCCTTGTTGG-3′ 1055-1032
cagA C2 (+)* 5′-GAATTGTCTGATAAACTTGAAA-3′ 2059-2080
cagA C2 (−)* 5′-TTTGCTTGCGTTACCTTGCTG-3′ 2306-2286
cagA C3 (−)* 5′-GCGTATGTGGCTGTTAGTAGCG-3′ 3210-3189
cagA 3′F1* 5′-AAACCCTGAGTGGCTCAAGCTC-3′ 3273-3294
a

*, primer used for sequencing.

b

Nucleotide positions in the HP0547 (cagA) gene of H. pylori 26695 (GenBank accession no. AE000569).

c

Product size of 3,576 bp.