TABLE 1.
Primera | Sequence | Corresponding DNA sequenceb |
---|---|---|
cagA L2 (+) | 5′-AAGGAGAAACAATGACTAACGAAACTATTG-3′ | −11-19c |
cagA L2 (−) | 5′-TCCTTTAAGATTTTTGGAAACCACCTTTTG-3′ | +4-3536 |
cagA B1 (−)* | 5′-CTGCAAAAGATTGTTTGGCAGA-3′ | 520-499 |
cagA M2 (+)* | 5′-ATACAAGGCTTACCGCCTG-3′ | 754-772 |
cagA S2* | 5′-GGCAATGGTGGTCCTGGAGCTAGGC-3′ | 976-1000 |
cagA M1 (−)* | 5′-GTAGCCACATTGTCGCCTTGTTGG-3′ | 1055-1032 |
cagA C2 (+)* | 5′-GAATTGTCTGATAAACTTGAAA-3′ | 2059-2080 |
cagA C2 (−)* | 5′-TTTGCTTGCGTTACCTTGCTG-3′ | 2306-2286 |
cagA C3 (−)* | 5′-GCGTATGTGGCTGTTAGTAGCG-3′ | 3210-3189 |
cagA 3′F1* | 5′-AAACCCTGAGTGGCTCAAGCTC-3′ | 3273-3294 |
*, primer used for sequencing.
Nucleotide positions in the HP0547 (cagA) gene of H. pylori 26695 (GenBank accession no. AE000569).
Product size of 3,576 bp.