TABLE 1.
Color detection for beta-hemolytic GBS strains by the use of three pigment-detecting broth mediaa
| Medium and test dateb | No. (%) of strains with pigment at indicated concn (CFU/ml) |
No. of isolates negative for pigment production and positive on BAP subculture at indicated concn (CFU/ml)/total no. of subcultured isolates |
||||
|---|---|---|---|---|---|---|
| After incubation at 24 h |
Only after incubation at 48 h |
|||||
| 102 | 101 | 102 | 101 | 102 | 101 | |
| Carrot (SBCB) | ||||||
| TD-1 | 45 (97.8) | 45 (97.8) | 1 (2.1) | 1 (2.1) | ||
| TD-2 | 46 (100) | 46 (100) | ||||
| TD-3 | 46 (100) | 45 (97.8) | 0 | 0/1 | ||
| Granada (IGLB) | ||||||
| TD-1 | 46 (100) | 46 (100) | ||||
| TD-2 | 46 (100) | 45 (97.8) | 0 | 0/1 | ||
| TD-3 | 46 (100) | 44 (95.7) | 1 (2.1) | 0/1c | ||
| TD-4 | 46 (100) | 43 (93.5) | 0 | 0/3 | ||
| NEL-GBS | ||||||
| TD-1 | 37 (80.4) | 38 (82.6) | 2 (4.3) | 4 (8.6) | 6/7c | 4/4 |
| TD-2 | 43 (93.4) | 43 (93.4) | 2 (4.3) | 2 (4.3) | 1/1 | 1/1 |
| TD-3 | 37 (80.4) | 37 (80.4) | 3 (6.5) | 4 (8.6) | 6/6 | 3/5 |
| TD-4 | 40 (86.9) | 39 (84.7) | 4 (8.6) | 3 (6.5) | 2/2 | 2/4 |
a
Forty-six strains were used.
b
TD-1, test date 1; TD-2, test date 2; TD-3, test date 3; TD-4, test date 4.
c
Subculture to BAP for one isolate that was negative after 24 h in broth was not determined.