FIG. 8.

PLCγ mediates plexin-B1-dependent cellular migration. Normal MCF-7 cells or MCF-7 cells stably expressing siRNA-insensitive mutants of wild-type plexin-B1 and PLCγ1 or their indicated mutants (A and C) were transfected with siRNA directed against the indicated targets (PLCγ indicates PLCγ1 plus PLCγ2) and kept at 0.5% FBS. Cells were then incubated in the absence (control) or presence of 150 nM Sema4D or 10% FBS. Sema4D-induced cellular migration (B and left panels of A and C) and RhoA activation (right panels of A and C) were analyzed as described in Materials and Methods. Control cells were stably transfected with the viral vector pLNCX2 alone. Data presented are representative of at least three independently performed experiments. Data of the analysis of migration are shown as means of triplicates (±standard errors of the means). **, P ≤ 0.01; ***, P ≤ 0.001.