FIG. 4.

Interference of HDV assembly in cells expressing CHC-targeted shRNA. COS7 cells were transfected with plasmid expressing shRNAs as indicated (A) or cotransfected with plasmids expressing HDAg-L, small HBsAg, and the shRNAs (B). Cells were harvested, and VLPs in the culture medium were collected for Western blot analysis with antibodies as indicated. Cells not expressing shRNA (−) and cells expressing shRNA against luciferase serve as controls. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.