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. 2009 Sep 23;83(23):12068–12083. doi: 10.1128/JVI.00963-09

FIG. 5.

FIG. 5.

Knockdown of Skp1 with siRNA disrupts binding of M-T5 to the SCF complex. (A) HEK 293 cells were transfected with either control (dark bars) or Skp1-specific (light bars) siRNA and the indicated (+) combination of plasmids. Harvested after 48 h, cell lysates were assayed for binding by AlphaScreen as described in the legend for Fig. 3. Knockdown of Skp1 protein expression by the siRNA treatment in HEK 293 cells was determined by Western blot analysis (inset). (B) The type II human cancer cell line 786-0 (56) was mock transfected or transfected with either control or Skp1- or CUL1-specific siRNA and, 24 h later, was infected with vMyx-gfp or vMyx-T5KOgfp at an MOI of 0.1. Viral focus formation was measured at 48 hpi by fluorescence microscopy. α, anti.