TABLE 1.
List of primers used to PCR amplify M-T5 constructs
| Primer | Sequence |
|---|---|
| MT5for | AAAGGATCCATGGATCTATACGGG |
| MT5rev | AAACTCGAGCGCGTGTATCTTTAC |
| ΔNfor | AAAGGATCCATGCGGGATACCCCCTTTCGC |
| ΔN-Ifor | AAAGGATCCATGGTTCGTGGTTCTCGTACG |
| ΔN-IIfor | AAAGGATCCATGGCGCCAGATGGACG |
| ΔN-IIIfor | AAAGGATCCATGCGGGGAACGGACGGATACG |
| ΔN-IVfor | AAAGGATCCATGCCAGACAAGACGTACGGG |
| ΔN-Vfor | AAAGGATCCATGGGATTACACGCGACC |
| ΔN-VIfor | AAAGGATCCATGGTGAACTTTCTGGGG |
| ΔN-VIIfor | AAAGGATCCATGTACACGAGGGCGTTGAACG |
| ΔILrev | AAACGAGAACCACGAACCGAACATAGTTCGTCCGG |
| ΔIRfor | AAACCGGACGAACTATGTTCGGTTCGTGGTTCTCG |
| ΔIILrev | AAACGTCCATCTGGCGCCATAGAACCACGAACTCCG |
| ΔIIRfor | AAACGGAGTTCGTGGTCCTATGGCGCCAGATGGACG |
| ΔI-IILrev | AAACGTCCATCTGGCGCCATCGAACATAGTTCGTCCGG |
| ΔI-IIRfor | AAACCGGACGAACTATGTTCGATGGCGCCAGATGGACG |
| ΔIII-Vlrev | AAAGGTCGCGTGTAATCCATCTGGCGCCATCGCG |
| ΔIII-Vrfor | AAACGCGATGGCGCCAGATGGATTACACGCGACC |
| ΔVI-VIILrev | AAACGTTCAACGCCCTCGTGTAGAGAGAGTTTTGTTCG |
| ΔVI-VIIRfor | AAACGAACAAAACTCTCTCTACACGAGGGCGTTGAACG |
| ΔPRANCrev | AAACTCGAGCCCAGGCGTGTCCAGCC |