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. 2009 Sep 23;83(23):12388–12398. doi: 10.1128/JVI.00304-09

FIG. 1.

FIG. 1.

Transactivation of p53 promoter by HCMV IE1-72 and IE2-86. (A) Western blots demonstrating expression of IE1-72, IE2-86, UL84, and p53 in HCMV-infected HEL cells harvested and lysed at the times (hours postinfection) indicated. M, mock infected. (B) Results of CAT assays demonstrating activation of p53 promoter in lysates from HEL cells harvested at the indicated times after HCMV infection. Bars indicate standard deviations. (C) CAT assays from lysates of Saos-2 cells transfected with combinations of target promoter construct, p53 promoter-CAT, and plasmids encoding wild-type p53, mutant p53 (p53mut), IE1-72, and IE2-86, as indicated. Values shown are means plus standard deviations for three experiments, each run with triplicate samples. pcDNA3, transfection with empty plasmid.