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. 2009 Sep 23;83(23):12388–12398. doi: 10.1128/JVI.00304-09

FIG. 4.

FIG. 4.

Both HCMV IE1-72 and IE2-86 interacted with HCMV pUL84. (A) Western blots demonstrating the presence of UL86 in complexes immunoprecipitated with anti-IE2-86 (upper panel) and anti-IE1-72 (lower panel) antibodies from lysates of HCMV-infected cells harvested at the indicated times postinfection. (B) Western blots (WB) demonstrating IE1-72 and IE2-86 immunoreactivity in complexes immunoprecipitated (IP) with anti-HCMV UL84 antibody from lysates of HCMV-infected HEL cells harvested at the indicated times postinfection. (C) Lysates of HCMV-infected cells harvested at the time indicated were immunoprecipitated first with anti-IE2 antibody, and the supernatant was subsequently immunoprecipitated with anti-UL84 antibody. Both immunoprecipitated complexes were subjected to Western blotting to detect the presence of IE1-72 with anti-IE1 antibody. α, anti; IgG, immunoglobulin G.