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. 2009 Sep 23;83(23):12388–12398. doi: 10.1128/JVI.00304-09

FIG. 6.

FIG. 6.

HCMV IE1-72, IE2-86, and UL84 downregulated p53-dependent gene transactivation in Saos-2 cells. (A) Diagram of target constructs showing wild-type (pG13CAT) and mutant (pMG15CAT) CAT reporter plasmids. WT* PRE, wild-type p53 responsive element with 13 copies of 5′-CCTGCCTGGACTTGCCTGG-3′ in plasmid pG13CAT (also called pSK-45-13-2-PyCAT); MUT* PRE, mutant PRE with 15 copies of 5′-CCTTAATGGACTTTAATGG-3′ in plasmid pMG15CAT (also called pSK89-15-1-PyCAT). (B and C) CAT assays of lysates from p53-negative Saos-2 cells cotransfected with combinations of expression vectors for IE1-72 and IE2-86 (B) and for IE2-86 and UL84 (C), along with wild-type p53 (p53WT) or mutant p53 (p53mut), as indicated, and with expression vectors for the target reporter constructs described in panel A, as indicated.