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. 2009 Sep 16;83(23):12651–12655. doi: 10.1128/JVI.01012-09

FIG. 1.

FIG. 1.

Arenavirus Z protein and its budding efficiency. (A) Schematic representation of Z proteins from LCMV, LASV, and TACV. G corresponds to the strictly conserved glycine residue found at position 2 of all known arenavirus Z proteins. The locations of bona fide PTAP (LASV), PPPY (LASV and LCMV), and YXXL (LASV and TACV) L-domain motifs are indicated, as well as that of the L-like domain motifs STAP (LCMV) and ASAP (TACV). (B) Budding activity of TACV Z protein. 293T cells were transfected with 0.25 μg of either pC-TACV-Z-HA or pC-LASV-Z-HA. At 36 h posttransfection, tissue culture supernatants were collected, and VLPs and total cell lysates were prepared as described previously (2). Levels of Z proteins in VLPs and cell lysates were determined by WB using an antibody to HA (sc-7392; Santa Cruz). The budding efficiency of LASV Z was set at 1.0. The data are averages and standard deviations from three independent experiments.