FIG. 6.

Virus replication is required for JNK activation. Primary astrocytes were infected with live or UV-inactivated MHV-A59 at an MOI of 5 or mock infected. At 24 h p.i., the cell lysates were isolated and used for detecting phosphorylated JNK (P-JNK) and c-Jun (P-c-Jun) or total JNK (T-JNK) by Western blot analysis using specific antibodies as described in the legend to Fig. 5. β-Actin was used as an internal control.