Skip to main content
. 2009 Sep 30;83(23):12535–12544. doi: 10.1128/JVI.01623-09

FIG. 1.

FIG. 1.

Effect of PGC1α and SHP expression on HBV biosynthesis in the human hepatoma cell line Huh7. Cells were transfected with the HBV DNA (4.1-kbp) construct alone (lane 1) or with the HBV DNA (4.1-kbp) construct plus the PGC1α and SHP expression vectors (lanes 2 to 16), as indicated. (A) RNA (Northern) filter hybridization analysis of HBV transcripts. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript was used as an internal control for RNA loading per lane. (B) Quantitative analysis of the 3.5-kb HBV RNA results from four independent experiments. Trend lines were calculated using linear regression analysis. (C) DNA (Southern) filter hybridization analysis of HBV replication intermediates. HBV RC DNA, HBV relaxed circular DNA; HBV SS DNA, HBV single-stranded DNA. (D) Quantitative analysis of the HBV replication intermediate results from four independent experiments. Trend lines were calculated using linear regression analysis.