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. 2009 Sep 30;83(23):12355–12367. doi: 10.1128/JVI.01593-09

FIG. 4.

FIG. 4.

Isotype and complement-dependent human antibody neutralization of VACV EV. (A) Titration of anti-B5 MAb h104 IgG1, IgG3, or IgG4 isotype by rB5 ELISA. (B to C) VACV EV neutralization activity with or without different MAb h104 isotypes at 10 μg/ml in the absence (B) or the presence (C) of 10% of rabbit complement. VACV EV alone (B) or plus complement (C) (—) was a negative control. The dashed line indicates 50% neutralization based on VACV EV alone (B) or in the presence of complement without antibody (C). (D and E) Titrated VACV EV neutralization activity of human anti-B5 MAbs in the absence (D) or the presence (E) of 10% of rabbit complement. All data are representative of three or more experiments.