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. 2009 Sep 30;83(23):12355–12367. doi: 10.1128/JVI.01593-09

FIG. 9.

FIG. 9.

VIG or plasma from individual human vaccinees directs complement lysis of VACV-infected cells. (A to C) Virus-infected Vero E6 cells stained with crystal violet at magnification ×40. VACV-infected cells were treated with VIG, plasma from a single human vaccinee (immune), or nonimmune human plasma alone (A to C, left) or with complement (+ C′) (A to C, right). (D and E) Quantitation of live cell numbers (cells/image field) after cell lysis in the presence of VIG (D) or human plasma (E). Destruction of VACV-infected cells was highly statistically significant in the presence of VIG plus complement (P < 0.02) or immune human plasma plus complement (P < 0.0006). No killing was observed for nonimmune human plasma in the absence or presence of complement (P ≫ 0.05; not significant). Error bars indicate the SEM for each group. One of two independent experiments is shown.