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Toxin A titers, expressed as absorbance units, were determined by ELISA. Toxin A production in overnight cultures of ΦCD119 lysogens was compared to parental strains. The ELISA signal was recorded as absorbance at 450 nm relative to the background signal, and the data shown are the mean plus standard error of three replicative samples. Experiments were repeated three times, and the figure represents the data from a single experiment.
