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. 2009 Sep 23;83(23):12415–12423. doi: 10.1128/JVI.01487-09

FIG. 3.

FIG. 3.

Genetic basis of the ts phenotype of BVDV TS2.7. (A) Genetic characterization of TS2.7. A schematic representation of the genome organization of the parental BVDV strain CP7 is shown on the top. Nucleotide sequencing of the entire genome of BVDV TS2.7 and subsequent comparison with the sequence of BVDV CP7 led to identification of five nucleotide changes at positions 1139, 3575, 4394, 10156, and 10542. The resulting four amino acid changes are indicated on the bottom. The mutation at position 4394 together with the resulting substitution of a tyrosine (Y) residue by a histidine (H) residue at position 1338 of the CP7 polyprotein is highlighted by a box. (B) The ts phenotype of BVDV mutant CP7-Y1338 carrying the point mutation at position 4394 in the CP7 genome. Plaque assays of cells infected with BVDV CP7-Y1338H at 33°C resulted in detection of plaques (top), while infection at 39.5°C led to production of foci (bottom). Plaques and foci were visualized by immunostaining 7 days after infection of cells.