FIG. 2.

Detection of PLMVd immunoprecipitated with eEF1A. eEF1A proteins were immunoprecipitated from healthy and infected peach leaf extracts, RNAs were extracted, and then PLMVd was amplified by RT-PCR and analyzed by 1% agarose electrophoresis. Lane 1, control RT-PCR amplification performed without nucleic acid (only water); lane 2, RT-PCR amplification performed using the healthy leaves, but without immunoprecipitation; lane 4, RT-PCR amplification performed after immunoprecipitation in the absence of eEF1A antiserum, using PLMVd-infected leaves; lanes 3 and 5, RT-PCR amplifications performed after immunoprecipitation in the presence of eEF1A antiserum, using healthy and PLMVd-infected leaves, respectively; lanes 6 and 7, RT-PCR amplifications performed using RNA extract from PLMVd-infected leaves and PLMVd synthesized by runoff transcription, respectively. Adjacent to the gel is a DNA molecular size marker, in bp.