TABLE 2.
Dissociation constants (Kd) and association rates (ka) for the interaction of eEF1A with various RNAsb
RNA species | EMSA Kd (nM) | Fluorescence spectroscopy ka (μM−1 s−1) |
---|---|---|
(−) PLMVd | 210 ± 31 | 420 ± 29 |
(+) PLMVd | 386 ± 49 | 342 ± 6 |
tRNAAla | >900 | 250 ± 30 |
CU27 | NDa | 178 ± 19 |
ND, not determined.
Data are means ± standard deviations.