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Western blot analysis of VZV IE63. Lysates of uninfected MeWo cells (lanes 1), VZV-infected MeWo cells (lanes 2), purified Flag-tagged IE63 expressed in E. coli (lanes 3), and purified Flag-tagged IE63 expressed in HEK 293 cells (lanes 4) were incubated with rAbs 63E4 (A) and 63P4 (B). Antibody 63E4 detected IE63 (∼38 kDa) only in mammalian cells, while antibody 63P4 detected IE63 in both mammalian and bacterial cells.
