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EBNA3C residues required for RBP-Jκ association. BJAB cells were transfected with 15 μg of the oriP plasmid expressing wt EBNA3C (E3C), EBNA3C mutants, or control GFP. At 48 h after transfection, protein complexes were immunoprecipitated (IP) with polyclonal rabbit sera raised against RBP-Jκ and Western blotted (IB) with anti-EBNA3C antibodies (A10 or F125P) or RBP-Jκ-specific rat monoclonal antibody (T6709). Arrowheads indicate the full-sized protein identified by the immunoblot.
