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. 2009 Sep 16;83(23):12027–12036. doi: 10.1128/JVI.00593-09

FIG. 1.

FIG. 1.

Modified DOX-regulated expression cassettes provides robust control of gene expression. (A) Schematic representations of the expression elements in ptTA-TRE-SIINFEKL-Luc, where a cassette expressing tTA under the control of the constitutive MCMV promoter was fused tail-to-tail with the TREminIL2-SIINFEKL-Luc expression cassette, and in ptTA-HS4-TRE-SIINFEKL-Luc, a derivative of ptTA-TRE-SIINFEKL-Luc in which an HS4 core insulator fragment was inserted between the two cassettes. (B) Plasmids containing the expression elements diagramed in panel A were individually transfected into BHK cells and cultured in the presence or absence of DOX (10 μg/ml), and luciferase activity was assayed. (C) An rHuAd5 vector (Ad-tTA-SIINFEKL-Luc) encoding the bifunctional, HS4-separated expression system diagramed in panel A was purified and tested at a range of multiplicities of infection on BHK cells in the presence or absence of DOX (10 μg/ml).