FIG. 3.

DOX treatment does not affect the magnitude of the CD8⁺ T-cell population produced by rHuAd5 vectors that employ constitutive promoters. C57BL/6 mice (n = 10) were immunized with 10⁷ PFU of Ad-SIINFEKL-Luc, and half of the mice were treated with DOX beginning at 7 days postimmunization (Dox d+7). The number of SIINFEKL-specific CD8⁺ T cells was assessed by tetramer staining of peripheral blood mononuclear cells at various times postimmunization. Each point represents the mean ± standard error of the mean for five mice.