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. 2009 Sep 16;83(23):12027–12036. doi: 10.1128/JVI.00593-09

FIG. 8.

FIG. 8.

Premature extinction of transgene expression results in increased frequencies of central memory cells. Mice were first immunized with Ad-tTA-SIINFEKL-Luc and were then put on DOX 13, 20, and 30 days later. Splenocytes were obtained 90 days after immunization and were examined for the expression of KLRG1, CD27, CD62L, and CD127. Antigen-specific CD8+ T cells were identified by tetramer staining. The histograms represent SIINFEKL-specific CD8+ T cells. (A and B) Frequencies of cells expressing CD62L and CD127, respectively. (C) Frequencies of effector (CD127− CD62L−), effector memory (CD127+ CD62L−), and central memory (CD127+ CD62L+) cells within the tetramer-positive CD8+ population. (D and E) Frequencies of cells expressing KLRG1 and CD27, respectively. Data reflect the means ± standard errors of the means for five mice. *, significantly different from the group receiving no DOX (P < 0.05).