FIG. 2.

Plaque morphologies and relative sizes of gK mutant and wild-type HSV-1(F) viruses. Vero cells were infected with an MOI of 0.001, and viral plaques were fixed with methanol and stained with anti-HSV antibodies as described in Materials and Methods. (A) Representative viral plaques of all gK mutant viruses and the HSV-1(F) wild-type virus are shown on both Vero (A to E) and VK302 (F to J) cells (constitutively expressing gK). (B) Bar graph showing the average plaque sizes of 70 randomly chosen viral plaques for each virus; error bars indicate the standard error (see Materials and Methods).