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. 2009 Sep 30;83(23):12301–12313. doi: 10.1128/JVI.01329-09

FIG. 3.

FIG. 3.

One-step replication kinetics of HSV-1(F) wild-type (wt) and gK mutant viruses. Vero cells were infected at either a low MOI (MOI of 0.1) or a high MOI (MOI of 2), and the numbers of infectious viruses produced were determined on VK302 cells at different times postinfection. At each time point, supernatants and infected cells were collected, and virus titers were determined separately. Viral titers after low-MOI infection are shown in panels A (intracellular) and B (supernatant). High-MOI titers are shown in panels C (intracellular) and D (supernatant). Viral titers obtained after low-MOI infections on VK302 cells are shown in panel E. Ratios of intracellular to extracellular viral titers obtained at 24 hpi are shown in panel F. gKins17, ΔgK recombinant virus created by inserting a kanamycin resistance cassette after aa position 17 within the gK signal sequence.