FIG. 7.

Cell surface expression of the gKa peptide. Vero cells were transfected with the gKa plasmid or the control plasmid gKa-R, and transfected cells were infected with the gKΔ31-68/gBΔ28syn virus. Cells were stained with either the anti-FLAG (α-FLAG) or the anti-HSV-1 (α-HSV) antibody at 30 hpi under either methanol-fixed (A) or live (B) conditions. The percentages of FLAG-positive live cells in gKa-transfected cells infected with either wild-type HSV-1(F) or the ΔgK or ΔUL20 mutant are shown as contour plots (C) and as bar graphs (D). SSC, side scatter.