FIG. 2.

UL142 remains endo H sensitive following synthesis. (A, B, and C) HeLa cells transfected with GFP-UL142 or mock transfected were labeled with [³⁵S]methionine and [³⁵S]cysteine for 20 min and then chased in unlabeled methionine and cysteine for 0, 45, 180, or 360 min. GFP-UL142 was immunoprecipitated using either rabbit anti-UL142 sera (A) or monoclonal anti-GFP antibody (B). Fractions of the eluted proteins were digested with endo H and analyzed by gel electrophoresis. GFP-UL142 remained endo H sensitive and had disappeared by the 360-min chase time point. (D) The control cell surface protein MOG 25.1, which was analyzed in parallel, acquired endo H resistance after the 45-min chase. IP, immunoprecipitant; +, present; −, absent.