Figure 1. In vitro differentiation potential of transduced aNSCs.

Modified neurospheres were dislodged and dissociated into single cells, then transferred into poly-l-lysine–precoated chamber slides and maintained in differentiation medium for 10 days. (A) aNSCs transduced with both vectors are GFP⁺ and exhibit neural cell morphology. IL-10 (red) was expressed in IL-10–aNSCs but not in GFP-aNSCs. (B) Double labeling with GFP and neural specific markers (red) indicate that aNSCs transduced with both vectors differentiated into NeuN⁺ neurons, NG2⁺ oligodendrocytes, GFAP⁺ astrocytes, or remained undifferentiated (nestin⁺), as verified by immunostaining and confocal microscopy. Original magnification, ×20 (A); ×65 (B). (C) Quantitative analysis. Symbols represent mean ± SEM of 4 independent experiments. *P < 0.05.