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. 2009 Nov 2;119(12):3678–3691. doi: 10.1172/JCI37914

Figure 3. Localization and migration of transplanted aNSCs in the CNS.

Figure 3

Mice treated with aNSCs i.v. at day 22 p.i. were sacrificed 2, 6, and 11 weeks p.t., and brains were harvested for immunohistology. The same region of the corpus callosum was examined in all groups, as shown in Supplemental Figure 3. (A and B) Transplanted aNSCs (green) were primarily confined to perivascular spaces 2 weeks (Ws) p.t., while the majority of these cells had migrated to the parenchyma by 6 weeks p.t. Blood vessels were stained with anti-laminin antibody (red). (C) GFP+ aNSCs remained in perivascular spaces of inflammatory areas, in which blood-borne CD45+ immune cells (red) that form the CNS inflammatory infiltrate persisted. (D) The majority of aNSCs in perivascular spaces remained positive for nestin (red). Nuclei in A, B, and C were stained with DAPI (blue). The high-magnification images represent the boxed regions (A, C, and D). Original magnification, ×20 (A and B); ×40 (C and D); ×65 (high-magnification images, A, C, and D). (E) Quantitative analysis of aNSCs that reached the parenchyma of EAE at 2, 6, and 11 weeks p.t. Symbols represent mean ± SD; n = 6–8 mice per group. *P < 0.05, **P < 0.01, comparisons between groups 2 weeks p.t. and groups at other time points; #P < 0.05, comparison of groups between 6 and 11 weeks p.t.