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. 2009 Nov 9;119(12):3797–3806. doi: 10.1172/JCI37976

Figure 7. Molecular features of DM1 reappear after withdrawal of Ro-31-8220.

Figure 7

(A) Western blot analysis of CUGBP1, phospho-PKCα/βII, total PKCα/βII, and connexin 43 in heart tissue from control mice (MCM) (lanes 1 and 2), mice treated with tamoxifen (TAM) (all 5 mice died within 3 weeks) (lanes 3–7), and mice treated with Ro-31-8220 for 43 days then taken off the drug for 22 days (Ro-31-820-STOP) (lanes 8–11). GAPDH was used as a loading control. (B) 2D/Western blot analysis of CUGBP1 in MCM, TAM, and Ro-31-8220-STOP groups. (C) Both CUGBP1- and MBNL1-specific splicing events were misregulated in heart tissues from Ro-31-8220-STOP animals. The number of animals per group for RT-PCR analysis is noted (mean ± SEM). *P < 0.05 versus MCM control.