(A) Nr0b2 mRNA levels in whole testes of P10 Nr0b2+/+ and Nr0b2L–/L– males exposed to 0.75 μg DES (n = 6), and in whole testes of 10-week-old males neonatally exposed to 0, 0.75, and 5 μg DES (n = 3–5). Values were normalized to β-actin. (B) Each male was bred with 5 C57BL/6J females to analyze the number of pups per litter. (C and D) Spermatozoa count in the tail of epididymis (C) and whole body weight as well as weights of testis, epididymis, seminal vehicles, and liver normalized to body weight (D) of 10-week-old Nr0b2+/+ and Nr0b2L–/L– males neonatally exposed to 0, 0.35, 0.5, 0.75, 1.5, and 5 μg DES (n = 6–14 per group). *P < 0.05 versus vehicle; #P < 0.05 versus Nr0b2+/+ given the same DES dose.