Skip to main content
. 2009 Nov 2;119(12):3752–3764. doi: 10.1172/JCI38521

Figure 5. Nr0b2 inhibits neonatal germ cell differentiation in response to DES.

Figure 5

Nr0b2+/+ and Nr0b2L–/L– mice were exposed to 0 or 0.75 μg DES. (A and B) Testicular mRNA expression of Oct3/4, Nanos3, Cyclin-d2, Stra8, Dmc1, and Cyclin-a1, normalized to β-actin levels, in P6 (A) or P10 (B) mice (n = 5–10 per group). (C) Immunoblot of activate caspase-3 on testicular protein extracts (n = 6 per group). Quantification of activate capase-3 protein accumulation relative to total caspase-3 is also shown. (D) Intratesticular testosterone levels in P10 mice (n = 10–15 per group). (E–G) mRNA expression of Star, Pem, and Osp (E); Nr3a1, Nr3a2, Nr3c4, Nr5a2, and Nr0b1 (F); and Insl3, Ren1, and Greb1 (G) in whole testes of P10 mice (n = 10–15 per group). Values were normalized to β-actin levels. Vehicle-treated mice were set at 100%. *P < 0.05 versus vehicle; #P < 0.05 versus Nr0b2+/+ given the same DES dose.