Skip to main content
. 2009 Nov 2;119(12):3752–3764. doi: 10.1172/JCI38521

Figure 6. Nr0b2 induces alterations of histone H3K9 marks through the inhibition of G9a, in response to DES.

Figure 6

Nr0b2+/+ and Nr0b2L–/L– mice were exposed to 0 or 0.75 μg DES. (A and B) Immunoblots of H3K9me1 and H3K9me2 performed on testis (A), and quantification of H3K9me2 and H3K9me2 accumulation, relative to total H3 (B), of P10 mice (n = 9 per group). (C) Testicular mRNA expression of G9a, normalized to β-actin levels, in whole testes of P6 and P10 mice (n = 10–15 per group). (D) Immunoblot of G9a performed on P10 mice (n = 9 per group). Quantification of G9a protein accumulation, relative to actin, is also shown. (E) Testicular mRNA expression of G9a target genes Akr1c13, Akr1c12, Chst11, Wfdc15a, Mei1, 290, 110, 291, and Iap, normalized to β-actin levels, in whole testes of P10 mice (n = 10–15 per group). Vehicle-treated mice were fixed at 100%. *P < 0.05 versus vehicle.