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. 2009 Nov 2;119(12):3626–3636. doi: 10.1172/JCI39374

Figure 5. Inhibition of PTEN expression by shRNA rescues migration/invasiveness and tumorigenicity in Bmi-1–silenced cells.

Figure 5

(A) Inhibiting PTEN expression by shRNAs results in activated Akt and rescue of Snail in Bmi-1–silenced CNE2 and HONE-1 cells, as determined by Western blotting. (B) The migration/invasiveness-inducing properties of PTEN shRNAs or a scrambled control shRNA in Bmi-1 knockdown cell lines (CNE2 and HONE-1) using a Matrigel-coated Boyden chamber as described in Figure 1D. Original magnification, ×400. Error bars represent SEM. Lane 1, scramble control shRNA; lane 2, Bmi-1 shRNA #2; lane 3, Bmi-1 shRNA #2 and PTEN shRNA #1; lane 4, Bmi-1 shRNA #2 and PTEN shRNA #2. (C) Mice were injected subcutaneously with cells (105 cells/mouse). Tumor volumes were measured as described in Methods and plotted as the mean ± SEM. The left panel displays tumor growth in nude mice injected with the 3 groups of cells (CNE2 scrambled control, CNE2 Bmi-1 shRNA #2, and CNE2 Bmi-1 shRNA #2/PTEN shRNA #2 cells).