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. 2009 Nov 16;119(12):3666–3677. doi: 10.1172/JCI39832

Figure 2. miR-2861 promotes BMP2-induced ST2 osteogenic differentiation.

Figure 2

(A) Northern blot analysis of miR-2861 levels in ST2 cells after transfection with pre–miR-2861 or miR-C. U6 was used as a loading control. (B) Overexpression of miR-2861 enhanced BMP2-induced ST2 osteogenic differentiation. ST2 cells were stably transfected with pre–miR-2861 or miR-C, then treated with BMP2 for 48 hours. ALP activity and osteocalcin secretion were determined. Data are shown as means ± SD. *P < 0.05 vs. miR-C, n = 5. (C) Microscopic view of the effects of miR-2861 on matrix mineralization in ST2 cells. Shown is a representative microscopic view at a magnification of ×200 of cells transfected with pre–miR-2861 or miR-C after 20 days of culture. Quantification of Alizarin Red S stain via extraction with cetyl-pyridinium chloride. Data are shown as means ± SD. *P < 0.05 vs. miR-C, n = 3. (D) Levels of Runx2 mRNA were determined using qRT-PCR and are shown as fold induction relative to control. Runx2 protein expression was determined by Western blot and expressed as densitometry of Runx2/β-actin. Data are shown as means ± SD. *P < 0.05 vs. miR-C, n = 3.