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. 2009 Nov 16;119(12):3666–3677. doi: 10.1172/JCI39832

Figure 6. Runx2 mutant rescues anti–miR-2861–inhibited ALP activity.

Figure 6

BMP2-induced ST2 cells were cotransfected with anti–miR-C or anti–miR-2861 and Myc-tagged WT type II Runx2 or a Runx2 mutant containing KR substitutions in the HDAC5 deacetylation targets (Runx2-KR-240/245/365/366 mutant). (A) Transfection of the Runx2-KR-240/245/365/366 mutant into BMP2-induced ST2 cells rescued the anti–miR-2861 inhibition of ALP activity. Data are shown as means ± SD. *P < 0.05 vs. anti–miR-2861 + WT Runx2, n = 5. (B) Transfection of the Runx2 mutant into BMP2-induced ST2 cells rescued the anti–miR-2861–inhibited Runx2 protein levels. The abundance of Runx2 or Runx2 mutant was determined by Western blot using an anti-Myc antibody.