Figure 3. Th22 cells are stable and cannot be deformed into other T cell phenotypes.

Freshly isolated CCR10⁺ T cells did not convert into another Th subset. Shown are intracellular stainings for CCR10⁺CD45RA^– cells after 5 days under nonpolarizing (A), Th1 (B), Th2 (C), Th17 (D), Th22 (E), and Treg (F) conditions and subsequent stimulation with PMA/ionomycin. Shown are dot plots for IL-17 and IFN-γ, for IL-22 and IL-17, and of IL-17^–IL-22⁺ gated cells (red outline) for IFN-γ and IL-4. Th22 cells are shown by red shading. Numbers indicate relative percentages per quadrant. Data are representative for 3 independent donors. Mean values and corresponding IL-22, TNF-α, IL-13, and IL-4 secretion are shown in Supplemental Figure 3.