Figure 5. Th22 cells are enriched in the epidermal compartment of the skin.

Skin biopsies were separated into dermal and epidermal parts by dispase treatment. Dermal and epidermal T cell lines were analyzed by flow cytometry. (A) Representative 3-color intracellular staining for IL-22, IL-17, and IFN-γ (on Il-17^–IL-22⁺ gated cells; red outline) of a separated ACD biopsy. Numbers indicate relative percentages in each quadrant. (B) Calculated mean percentage of total IL-22⁺ cells in dermis and epidermis (n = 4 each of AE and ACD). Lines within boxes denote means; box upper and lower bounds indicate SD; and whiskers indicate minimum and maximum values. *P < 0.05.