Figure 3. Gsk3-depleted HSCs are functionally deficient.

(A) Limiting dilution experiments were performed with 4 doses (x axis) of GFP⁺ test BM from control and Gsk3-rnai primary recipients (4 donors per group) combined with a fixed 2 × 10⁵ unlabeled competing cells transplanted into groups of at least 5 recipients per dose. Chimerism at 4 months after transplantation for each dose is represented as the percentage of GFP⁺ cells in BM for control and Gsk3-rnai. (B) Percent donor-derived immunophenotypic HSCs/HPCs (as GFP⁺ LSK cells) in the 1 × 10⁶ test cell group 4 months after transplantation. (C) Absolute number of donor-derived immunophenotypic HSCs/HPCs (as GFP⁺ LSK cells) in the 1 × 10⁶ test cell group 4 months after transplantation.