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. 2009 Oct 7;83(24):13019–13025. doi: 10.1128/JVI.00638-09

TABLE 1.

Novel viral sequences with similarity to ASFV

Sample Sequencea Total readsb ASFV genec E valued % Identitye Protein description (reference[s]) Accession no.f
Human AFI and NS AFI-1 23,224 G1211R 1e−10 64 DNA polymerase (15, 19) FJ957903
NS-1.1 83,028 B354L 3e−11 42 ATPase (10) FJ957904
NS-1.2 14,737 NP1450L 2e−13 45 RNA polymerase, largest subunit (27) FJ957905
NS-2.1 42,635 M1249L 2e−10 36 Unknown FJ957906
NS-2.2 3,870 P1192R 5e−12 37 Topoisomerase II (3, 8) FJ957907
NS-3 2,602 C962R 7e−12 38 Primase or ATPase (10, 12) FJ957908
Sewage fractions SF-3g† 78,573 G1211R 2e−26 51 DNA polymerase (15, 19) FJ957909
SF-6g† 6,633 G1211R 1e−25 51 DNA polymerase (15, 19) FJ957910
SF-8.1# 33,028 D250R 4e−14 60 NTPh pyrophosphohydrolase (10) FJ957911
SF-8.2 33,028 EP1242L 1e−19 45 RNA polymerase, subunit 2 (27) FJ957912
SF-8.3 33,028 EP364R 5e−9 39 Nuclease (11) FJ957913
SF-8.4§ 33,028 EP424R 5e−9 58 RNA methyltransferase (11) FJ957914
SF-8.5 33,028 F1055L 3e−18 32 Helicase (23) FJ957915
SF-8.6g 33,028 F1055L 6e−8 50 Helicase (23) FJ957916
SF-8.7† 33,028 G1211R 5e−23 49 DNA polymerase (15, 19) FJ957917
SF-8.8 33,028 G1340L 1e−8 27 Unknown FJ957918
SF-8.9¶ 33,028 H339R 5e−13 44 Unknown FJ957919
SF-9.1* 13,968 C962R 4e−10 40 Primase or ATPase (10, 12) FJ957920
SF-9.2 13,968 CP2475L 5e−10 41 220-kDa polyprotein (21) FJ957921
SF-9.3§ 78,307 EP424R 2e−11 44 RNA methyltransferase (11) FJ957922
SF-10 16,843 C475L 9e−14 46 Polyadenylate polymerase (6) FJ957923
Unassigned UA.1g NA B646L 1e−11 44 Major capsid protein (13) FJ957924
UA.2g NA B962L 7e−37 50 Helicase (28) FJ957925
UA.3 NA C475L 1e−10 31 Polyadenylate polymerase (6) FJ957926
UA.4 NA C475L 8e−9 37 Polyadenylate polymerase (6) FJ957927
UA.5 NA C962R 6e−38 38 Primase or ATPase (10, 12) FJ957928
UA.6* NA C962R 1e−17 32 Primase or ATPase (10, 12) FJ957929
UA.7g NA CP2475L 2e−15 30 220-kDa polyprotein (21) FJ957930
UA.8# NA D250R 5e−14 60 NTP pyrophosphohydrolase (10) FJ957931
UA.9g NA EP1242L 1e−20 44 RNA polymerase, subunit 2 (27) FJ957932
UA.10g† NA G1211R 2e−26 51 DNA polymerase (15, 19) FJ957933
UA.11 NA G1211R 1e−15 41 DNA polymerase (15, 19) FJ957934
UA.12 NA G1340L 6e−15 32 Unknown FJ957935
UA.13 NA G1340L 1e−28 50 Unknown FJ957936
UA.14¶ NA H339R 8e−12 35 Unknown FJ957937
UA.15 NA M448R 2e−9 34 Unknown FJ957938
a

Sets of sequences whose members were similar to the same regions of the ASFV genome and had significant overlap and nucleotide sequence identity with each other are indicated by the same symbols (†, #, §, *, or ¶).

b

The total number of reads generated from each sample is given for each sequence whose parent sample is known. For samples that were sequenced in more than one sequencing run, the total number of reads for the run in which the indicated novel sequence was identified is given. NA, not applicable.

c

ASFV genes to which the novel viral sequences have sequence similarity, as determined using tBLASTx (1). ASFV genes are listed using nomenclature for the BA71V strain (29).

d

E value for tBLASTx comparison of the novel viral sequences to the ASFV BA71V complete genome (29).

e

Amino acid identity between the novel viral sequences and corresponding sequences in the ASFV BA71V strain within the region of similarity identified using tBLASTx.

f

GenBank accession numbers.

g

Sequence was assembled from two or more overlapping reads.

h

NTP, nucleoside triphosphate.