TABLE 1.
Numbers (and percentages) of different types of mutations found within 13 field isolates of ToCV
| Type of mutation | RNA1 |
RNA2 |
Total |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| RdRp |
Hsp70h |
CP |
CPm |
|||||||
| n | % | n | % | n | % | n | % | n | % | |
| A→C | 0 | 0.0 | 0 | 0.0 | 0 | 0.0 | 2 | 6.9 | 2 | 1.2 |
| A→G | 6 | 8.7 | 1 | 2.4 | 1 | 3.8 | 3 | 10.4 | 11 | 6.7 |
| A→U | 4 | 5.8 | 1 | 2.4 | 2 | 7.7 | 1 | 3.5 | 8 | 4.9 |
| G→A | 10 | 14.5 | 6 | 14.6 | 2 | 7.7 | 5 | 17.2 | 23 | 13.8 |
| G→C | 3 | 4.3 | 1 | 2.4 | 1 | 3.8 | 1 | 3.4 | 6 | 3.6 |
| G→U | 3 | 4.3 | 3 | 7.3 | 7 | 27.0 | 3 | 10.4 | 16 | 9.7 |
| C→A | 3 | 4.3 | 1 | 2.4 | 0 | 0.0 | 1 | 3.5 | 5 | 3.0 |
| C→G | 0 | 0.0 | 0 | 0.0 | 1 | 3.8 | 0 | 0.0 | 1 | 0.6 |
| C→U | 11 | 15.9 | 6 | 14.6 | 2 | 7.7 | 3 | 10.4 | 22 | 13.3 |
| U→A | 0 | 0.0 | 1 | 2.4 | 1 | 3.8 | 1 | 3.5 | 3 | 1.8 |
| U→C | 20 | 29.0 | 12 | 29.3 | 6 | 23.1 | 5 | 17.2 | 43 | 26.1 |
| U→G | 5 | 7.2 | 2 | 4.9 | 0 | 0.0 | 2 | 6.9 | 9 | 5.5 |
| Substitutions | 65 | 94.2 | 34 | 82.9 | 23 | 88.5 | 27 | 93.1 | 149 | 90.3 |
| Insertions | 1 | 1.4 | 1 | 2.4 | 0 | 0.0 | 0 | 0.0 | 2 | 1.2 |
| Deletions | 3 | 4.3 | 6 | 14.6 | 3 | 11.5 | 2 | 6.9 | 14 | 8.5 |
| Total | 69 | 100 | 41 | 100 | 26 | 100 | 29 | 100 | 165 | 100 |
| Transitions | 47 | 72.3 | 25 | 72.7 | 11 | 48.0 | 16 | 59.3 | 99 | 66.4 |
| Transversions | 18 | 27.7 | 9 | 27.3 | 12 | 52.0 | 11 | 40.7 | 50 | 33.6 |
| Nonsynonymous | 23 | 35.4 | 25 | 74.3 | 15 | 65.2 | 18 | 67.0 | 81 | 54.4 |
| Synonymous | 42 | 64.6 | 9 | 25.7 | 8 | 34.8 | 9 | 33.0 | 68 | 45.6 |
| Stop codons | 3 | 4.3 | 0 | 0.0 | 3 | 11.5 | 0 | 0.0 | 6 | 3.6 |